Mapping of Myxococcus xanthus social motility dsp mutations to the dif genes.
نویسندگان
چکیده
Myxococcus xanthus dsp and dif mutants have similar phenotypes in that they are deficient in social motility and fruiting body development. We compared the two loci by genetic mapping, complementation with a cosmid clone, DNA sequencing, and gene disruption and found that 16 of the 18 dsp alleles map to the dif genes. Another dsp allele contains a mutation in the sglK gene. About 36.6 kb around the dsp-dif locus was sequenced and annotated, and 50% of the genes are novel.
منابع مشابه
Myxococcus xanthus dif genes are required for biogenesis of cell surface fibrils essential for social gliding motility.
Myxococcus xanthus social (S) gliding motility has been previously reported by us to require the chemotaxis homologues encoded by the dif genes. In addition, two cell surface structures, type IV pili and extracellular matrix fibrils, are also critical to M. xanthus S motility. We have demonstrated here that M. xanthus dif genes are required for the biogenesis of fibrils but not for that of type...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 184 5 شماره
صفحات -
تاریخ انتشار 2002